Wajeed Khan
A unicellular microalga known as heterosigma akashiwo has the potential to kill large numbers of both wild and farmed fish globally, causing significant economic losses. Environmental factors including salt, light, and temperature had a big impact on how H. akashiwo bloomed and how deadly it was. While one-factor-at-a-time (OFAT) approaches, which only alter one variable at a time while holding others constant, were used in earlier studies, a design of experiment (DOE) approach, which is more precise and efficient, was used in the current study to examine the simultaneous effect of three factors and their interactions. To examine the impact of salinity, light intensity, and temperature on the toxicity, lipid production, and protein production of H. akashiwo, the study used a central composite design (CCD). In contrast to standard approaches that use the entire organism, a yeast cell assay was created to evaluate toxicity and provides quick and convenient cytotoxicity evaluations using a lesser number of samples. According to the results, H. akashiwo is most hazardous at 25 °C, 17.5 % salinity, and 250 mol photons per square metre per second of light. At 25 °C, a salinity of 30, and a light intensity of 250 mol photons m2 s1, the highest concentrations of lipid and protein were discovered. Warm water mixing with river input that is lower in salinity hence has the potential to improve H. akashiwo toxicity, which is consistent with environmental studies linking warm summers to situations with heavy runoff that pose the biggest threat to aquaculture enterprises.
KeywordsHeterosigma akashiwo; Toxicity; Saccharomyces cerevisiae; Salinity; Light
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